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Free MOTS-C reconstitution calculator. Enter vial size (5-10 mg), BAC water volume, and dose to get concentration, syringe units, and doses per vial. Includes bolus, intermittent, and low-dose daily protocols plus exercise-timing guidance. Verify MOTS-C quality with independent lab test data on Peptigrity.
A 10 mg vial of MOTS-c reconstituted with 2 mL of bacteriostatic water gives a concentration of 5.0 mg/mL, or 50 mcg per unit on a U-100 syringe. For a 5 mg dose, draw the syringe to 100.0 units (1.00 mL). At that dose the vial provides about 2 doses.
Starts at a 10 mg vial and 5 mg; 5 mg is the amount this page's own reference tables use. The calculator picks the water so your dose lands on a line of your syringe — or set your own. Arithmetic only, not a recommendation.
How many mg is on the label?
Bacteriostatic water — not sure how much? Let the calculator pick.
How much per shot?
5 mg = 5,000 mcg
Pick the one in your hand.
Not sure? Look at the biggest number printed on the barrel — 30, 50 or 100.
Check the box says U-100. Syringes for pet insulin (U-40) look the same but their lines measure 2.5× more liquid. This calculator is for U-100 syringes only.
Each line on this syringe = 2 units = 100 mcg.
Easy check: 10 units = 500 mcg · 20 units = 1 mg
| Step | Dose | Draw on a 1 mL syringe | Doses per vial |
|---|---|---|---|
| Low-dose daily | 1 mg | 20 units | 10 |
| Daily (high) | 2 mg | 40 units | 5 |
| Intermittent | 5 mg | 100 units | 2 |
| Bolus (max) | 10 mg | Exceeds syringe — split the dose or use less water | 1 |
Tap any row to load that dose into the calculator above.
Log it week by week in the free peptide tracker and check your vial's lot against lab tests on file. Private until you publish.
MOTS-C is a mitochondrial-derived peptide (MDP) - a 16-amino-acid signalling molecule encoded within the 12S rRNA gene of mitochondrial DNA. Its primary mechanism is AMPK activation, making it one of the few peptides characterised as an “exercise mimetic.” Doses per administration are high relative to other research peptides.
| Parameter | Value |
|---|---|
| Dose | 5-10 mg per injection |
| Frequency | 1× weekly |
| Duration | 8-12 weeks |
| Timing | Morning, 30-60 min before exercise |
| Notes | Most closely mirrors preclinical dosing; large draw volume |
| Parameter | Value |
|---|---|
| Dose | 5 mg per injection |
| Frequency | 2-3× weekly (e.g., Mon/Wed/Fri) |
| Duration | 6-8 weeks |
| Timing | Morning, before activity |
| Notes | Higher weekly total; used by those seeking more consistent AMPK activation |
| Parameter | Value |
|---|---|
| Dose | 1-2 mg daily |
| Frequency | 1× daily |
| Duration | 8-12 weeks |
| Timing | Morning |
| Notes | Highest total consumption; anecdotally gentler onset |
MOTS-C has high per-dose requirements - a single 5 mg dose uses half of a 10 mg vial. Choose BAC water volume carefully to keep draw volumes manageable.
| Vial | BAC Water | Conc. | 5 mg Draw | 10 mg Draw | Doses (5 mg) |
|---|---|---|---|---|---|
| 10 mg | 1 mL | 10 mg/mL | 50 units | 100 units (full) | 2 |
| 10 mg | 2 mL | 5 mg/mL | 100 units (full) | N/A (exceeds syringe) | 2 |
| 5 mg | 0.5 mL | 10 mg/mL | 50 units | N/A | 1 |
| 5 mg | 1 mL | 5 mg/mL | 100 units (full) | N/A | 1 |
Common: 10 mg vial + 1 mL BAC water = 10 mg/mL. A 5 mg dose = 50 units (half a syringe). Each 10 mg vial = exactly 2 bolus doses. For reconstitution technique, see reconstituting peptides step by step.
| Timing | Rationale | Community Usage |
|---|---|---|
| 30-60 min before exercise | AMPK activation may synergise with exercise-induced AMPK | Most common |
| Morning, non-exercise days | Maintains consistent administration schedule | Common for daily protocols |
| Evening | - | Uncommon - some report energy increase that may affect sleep |
MOTS-C activates AMPK - the same pathway triggered by exercise and metformin - which regulates glucose uptake, fatty acid oxidation, and mitochondrial biogenesis. Preclinical studies in mice showed MOTS-C prevented high-fat-diet-induced obesity and improved insulin sensitivity. Human data is extremely limited; plasma MOTS-C levels have been correlated inversely with BMI in observational studies, but no controlled human weight loss trials have been published.
| Property | MOTS-C | Semaglutide | Tesamorelin |
|---|---|---|---|
| Class | Mitochondrial-derived peptide | GLP-1 receptor agonist | GHRH analogue |
| Primary mechanism | AMPK activation | GLP-1R agonism, appetite suppression | GH release → lipolysis |
| FDA approved | No | Yes (obesity, T2DM) | Yes (HIV lipodystrophy) |
| WADA status | Prohibited (S2) | Not prohibited | Prohibited (S2) |
| Typical dose | 5-10 mg weekly | 0.25-2.4 mg weekly | 2 mg daily |
| Human trials | Very limited | Extensive | Moderate |
MOTS-C is classified under S2 - Peptide Hormones, Growth Factors, Related Substances and Mimetics on the WADA Prohibited List. It is banned both in-competition and out-of-competition. Athletes subject to anti-doping regulations should not use MOTS-C. Detection methods based on mass spectrometry can identify MOTS-C in urine and blood samples.
MOTS-C is a 16-amino-acid peptide with a molecular weight of approximately 2,174.7 Da. Check the CoA for ≥98% HPLC purity and mass spectrometry confirming the expected molecular weight. Cross-reference with independent lab test results. For CoA interpretation, see red flags in peptide certificates of analysis.
At 10 mg/mL (10 mg vial + 1 mL BAC water): 5 mg = 50 units. At 5 mg/mL (10 mg + 2 mL): 5 mg = 100 units (a full syringe). Use 1 mL BAC water for manageable draws.
Three protocols exist: weekly bolus (5-10 mg once), intermittent (5 mg 2-3× weekly), and low-dose daily (1-2 mg). The bolus protocol most closely mirrors preclinical dosing.
Yes. MOTS-C is on the WADA Prohibited List (S2 - Peptide Hormones). Prohibited in-competition and out-of-competition. Athletes subject to testing should not use MOTS-C.
Morning, 30-60 minutes before exercise is the most common timing. MOTS-C activates AMPK, which may synergise with exercise. Some users report increased energy that could interfere with sleep if taken in the evening.
Bolus (5 mg weekly, 8 weeks) = 4 × 10 mg vials. Intermittent (5 mg 3×/week, 6 weeks) = 9 vials. Daily (2 mg, 8 weeks) = ~12 vials. MOTS-C is one of the highest-consumption peptides by total milligrams.
Preclinical data shows MOTS-C prevented obesity in high-fat-diet mouse models and improved insulin sensitivity via AMPK activation. Human weight loss trials have not been published. It is not comparable to FDA-approved GLP-1 agonists like semaglutide.